This paper describes the development of BOLE, a MATLAB-based interactive learning environment, that facilitates the process of learning bio-inspired optimization algorithms, and that is dedicated exclusively to unmanned aerial vehicle path planning. As a complement to conventional teaching methods, BOLE is designed to help students consolidate the concepts taught in the course and motivate them to explore relevant issues of bio-inspired optimization algorithms through interactive and collaborative learning processes. BOLE differs from other similar tools in that it places greater emphasis on fundamental concepts than on complex mathematical equations. The learning tasks using BOLE can be classified into four steps: introduction, recognition, practice, and collaboration, according to task complexity. It complements traditional classroom teaching, enhancing learning efficiency and facilitating the assessment of student achievement, as verified by its practical application in an undergraduate course "Bio-Inspired Computing." Both objective and subjective measures were evaluated to assess the learning effectiveness.