Beliefs influence teacher decision in the classroom. Because of this reason, understanding teachers' beliefs is important. It is also critical to study teachers' beliefs who integrate science in the classroom. In this study, the effects of microteaching with lesson study approach on preservice science teachers' beliefs about learning and teaching have been analyzed. Teachers' Beliefs Interview (TBI) has been used for determining the development of preservice science teachers' beliefs about learning and teaching. At the end of the study, it was determined that through microteaching with lesson study approach, preservice science teachers' beliefs improved in a positive way that their beliefs significantly changed toward more student-centered. The findings from this study support the notion that application micro teaching with lesson study in Science Teaching Method Course has positive impact on preservice teachers' beliefs.