Environmental Monitoring is a basic and comprehensive course for students majoring in environmental sciences and engineering. Based on the characteristics of this course, a new teaching mode in application of practical talents training in Environmental Monitoring Curriculum teaching mode is proposed including the new scheme of training applied talents, the new practical curriculums of developing innovative ability, the diversified practice platform for training talents, the extracurricular innovation experiment , the diversified social practice, the teaching reform project and results summary. It shows that the new teaching mode can effectively improve students' ability in comprehension, operation, experiment, innovation and practice. This is a beneficial exploration for achieving the target of cultivating students with professional skills and quality.