This qualitative study explores the experiences of six students enrolled in a special topics biology class that exclusively used primary literature as course material. Nature of science (NOS) conceptions have been linked to students' attitudes toward scientific subjects, but there has been little research specifically exploring the effects of primary literature use on NOS conceptions. To explore these effects, we used both written responses to an established and validated NOS survey taken at the beginning and end of the course and reflective essays in which students described in detail their experiences with using primary literature. The results indicate positive gains in various aspects of NOS conceptions as well as increased confidence with approaching original research. We conclude by suggesting that future research should focus on how primary literature use affects nature of science conceptions. We also suggest the expanded use of primary literature in biology education.