In this observational study, male and female professional caregivers' (n = 42) levels of sensitivity and stimulation toward three-year-old children (n = 42) were observed in a semi-structured play situation. Further, sex roles of male and female caregivers were examined with the Bem Sex Role Inventory (BSRI). Male and female caregivers showed the same levels of attention, sensitivity, and stimulation toward boys and girls. Furthermore, all caregivers were classified as feminine or androgynous, but not masculine. Only for female caregivers was a positive relation found between higher levels of femininity and more stimulation of girls. Taken together, these findings provide evidence that male and female staff in childcare share similar interaction styles and sex roles. However, our findings suggest that caregivers' sex roles should be included in future research as well as in the debate on men in childcare.