This article describes the results of a study that investigated the use of the dissection of organs in anatomy and physiology classes in Slovenian lower and upper secondary schools. Based on a sample of 485 questionnaires collected from Slovenian lower and upper secondary school students, we can conclude that dissection of mammalian organs during the courses on Human Anatomy would be a preferred activity for the majority of them. Opinions on such practices are positive, and only a minority of students would prefer to opt out. However, the practice is performed only occasionally in regular classes, or even omitted, and a number of students never participate in it. According to the results, we can suggest the dissection of mammalian organs in combination with alternatives, such as 3D models and virtual laboratories, as a preferred strategy to increase knowledge of anatomy and to raise interest in science. However, students should know that the organs they are dissecting were dedicated to human consumption, or are waste products in these processes. Opt-out options should be provided for those who do not want to participate in such activities.