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ERIC Number: EJ1136311
Record Type: Journal
Publication Date: 2017-Mar
Pages: 5
Abstractor: As Provided
ISSN: ISSN-0021-9584
Utilizing Mechanistic Cross-Linking Technology to Study Protein-Protein Interactions: An Experiment Designed for an Undergraduate Biochemistry Lab
Finzel, Kara; Beld, Joris; Burkart, Michael D.; Charkoudian, Louise K.
Journal of Chemical Education, v94 n3 p375-379 Mar 2017
Over the past decade, mechanistic cross-linking probes have been used to study protein-protein interactions in natural product biosynthetic pathways. This approach is highly interdisciplinary, combining elements of protein biochemistry, organic chemistry, and computational docking. Herein, we described the development of an experiment to engage undergraduate students in multidisciplinary research that leverages mechanistic cross-linking probes to study protein conformations and protein-protein interactions. This experiment provides students with a platform to learn chemoenzymatic synthesis, polyacrylamide gel electrophoresis, biochemical assays, and computational docking, all while exploring a contemporary biochemical topic.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: National Institutes of Health (DHHS); National Science Foundation (NSF)
Authoring Institution: N/A
Grant or Contract Numbers: R15GM120704; R01GM095970; IOS1516156