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ERIC Number: EJ1136310
Record Type: Journal
Publication Date: 2017-Mar
Pages: 5
Abstractor: As Provided
Reference Count: 24
ISBN: N/A
ISSN: ISSN-0021-9584
Unboiling an Egg: An Introduction to Circular Dichroism and Protein Refolding
Hoben, John P.; Wang, Jianing; Miller, Anne-Frances
Journal of Chemical Education, v94 n3 p356-360 Mar 2017
A signature of life is that biological molecules occur as a single stereoisomer with respect to each chiral center, and this is echoed in the structures of biological polymers. Thus, circular dichroism (CD) has emerged as a hallmark of biomolecular structures. The tangible and familiar phenomenon of raw egg white's conversion from transparent fluid to rubbery white solid, upon boiling, was exploited to relate protein molecular structure measured by CD, to macroscopic material properties. Specifically, students employed a denaturant solution to dissolve hard-boiled egg white back into solution, and then upon dilution of the denaturant recovered CD signatures of structure. This experiment is adaptable to students from high school to early in graduate school, and provides an accessible introduction to the use of CD for characterizing protein secondary structure. It is a class favorite due to its demonstration of the surprising possibility of reversing the protein aggregation that underlies the solidity of hard-boiled egg white.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Secondary Education; Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: US Department of Energy, Office of Basic Energy Sciences (BES)
Authoring Institution: N/A
Grant or Contract Numbers: DESC0012518