The aim of this study is to determine the mediating role of prejudice in the relationship between the cultural intelligence of school principals and the level of entrepreneurship. The design of this study was classified as correlational survey research. This study was designed by quantitative research method. The universe of this study constitutes 642 School Principals. In this study, the entire universe was reached and a "complete counting" technique was used. Relations between the variables of this study were examined by correlation and hierarchical regression analysis. Findings have shown that cultural intelligence is positively related to prejudice and entrepreneurship. As a result of the hierarchical regression analysis, the prejudice has been completely mediated-agent between the cultural intelligence and the level of entrepreneurship. The positive relationship between cultural intelligence and prejudice has been identified for the first time and this finding contributes exceptional data for literature. This research is also particularly important in that it is the first study to examine the relationship between the level of entrepreneurship, the prejudice, and the cultural intelligence. The findings are helpful to the educational and organizational literature, but in practice, they will provide strategic alternatives for school principals to engage in entrepreneurial activities.