Childhood obesity and the decline of physical activity are real concerns for physical educators. Physical educators are in a key position to positively affect physical activity among youth at risk for obesity; however, the presence of an anti-fat bias may inhibit their ability to effectively do so. By addressing anti-fat bias, not only in physical education teacher education curricula but also with current physical educators, these individuals may be better prepared to meet the needs of students who are overweight, thus, hopefully resulting in increased student motivation and participation not only in physical education class but in those students choosing to be active for their lifetime. The purpose of this study was to determine whether a brief obesity awareness intervention could ameliorate anti-fat biases among preservice physical education teachers and, consequently, affect expectations of performance and ability as well as intentions to comply with recommended best practice.