The high level of overweight and obesity in children and adolescents has been termed a public health crisis and is associated with low levels of physical activity (PA). Experts recommend that youth engage in 60 minutes or more of moderate to vigorous PA per day, yet two-thirds of adolescents do not obtain this goal. Schools have been identified as locations in which PA promotion should occur, but there are limited opportunities for adolescents to enhance their PA during school hours. The present study used stationary bicycle desks (FitDesk) in a secondary school to investigate whether these desks enhanced student PA over an academic year. Results indicated that the students on the bicycle desks spent less relative time in sedentary activity, spent more time in moderate-to-vigorous physical activity (MVPA), and improved each of the PA outcomes compared to the control group from Assessment Period 1 to Period 2. Students and teachers noted both advantages and limitations of using the desks during the school day but overall were enthusiastic about the use of FitDesks in classrooms. Implications for enhancing activity during the school day for occupational therapists and limitations of using the stationary bike desks are discussed.