Cultural integration can be used as an effective learning practice in contexts of English as Foreign Language (EFL) classrooms. The present study aimed at investigating the effect of cultural integration on the development of Iranian EFL upper-intermediate learners' listening comprehension. To this end, fifty-two upper-intermediate EFL learners were selected based on the Quick Placement Test, developed by Oxford University Press and University of Cambridge Local Examinations Syndicate (2012). These participants were randomly assigned into experimental (N = 26) and control (N = 26) groups. T-test analysis indicated significant effects of cultural integration on the development of listening comprehension on upper-intermediate EFL learners. The findings offer pedagogical implications for integrating First Language (L1) culture in EFL listening comprehension classrooms.