The aim of present study was to investigate whether the mental well-being of health care workers associated with gender, age and marital status. Data were collected from 115 health care workers (doctors, physiotherapist, nurses, etc.) from a Turkish Medical Research and Application Center. They completed a demographic information form and the Mental Well-being Scale. Independent samples t-test and one-way analysis of variance (ANOVA) were used for data analyses. Results of current study showed that mental well-being levels of Turkish health care workers did not correlate with gender, age, or marital status. Future studies may investigate other psychological variables in order to more comprehensive understanding of mental well-being among health care workers.