Physical separation of the Great Lakes and Mississippi River basins has been identified as the most effective method for preventing the transfer of aquatic nuisance species, particularly Asian carp, from the Mississippi River Basin to the Great Lakes. The U.S. Army Corps of Engineers selected Extension to conduct a study of a key stakeholder group, Great Lakes charter captains, as a first step in assessing public opinion on the issue. Results reveal that the charter captains overwhelmingly support basin separation. Expanded educational outreach related to aquatic nuisance species, basin separation, and so forth would improve the ability of clientele to make informed decisions regarding separation of the basins.