Introduction: This paper presents the findings from a project that investigated the lived experiences of library and information professionals in relation to evidence-based practice within an Australian public library. Method: The project employed ethnography, which allows holistic description of people's experiences within a particular community or cultural setting. A member of the research team visited a public library regularly over a six month period. Data collection comprised interviews, observation and document analysis. A field journal was also maintained where daily activities and interactions were recorded. Analysis: Ethnography involves many levels of iterative analysis. Data extracts were identified, grouped and further refined whilst maintaining the context of the whole experience of the culture. Results: Evidence-based practice is experienced in the library through four interconnected and interdependent cultural orientations: (i) culture of valuing; (ii) culture of being; (iii) culture of learning; and (iv) culture of leading. These orientations represent not only how evidence-based practice is experienced in the library but also how it has been enabled. Conclusions: Context or environment is significant in terms of library and information service professionals' understanding of evidence-based practice. Evidence-based practice can be beneficial to all such professionals, as it builds professional knowledge and deeper understandings of practice at all levels of professional experience. [Paper presented at the Information Seeking in Context (ISIC): The Information Behaviour Conference, Part 1 (11th, Zadar, Croatia, September 20-23, 2016).]