The effects of technological developments occurred new requirements in educational area. Today's teachers should know the content knowledge they teach, have pedagogical knowledge about teaching and learning methods and besides should use the technological tools effectively. Depending on these, new concepts such as Technological Pedagogical Content Knowledge, Web Pedagogical Content Knowledge occurred. With this study it was aimed to determine self-efficacy perception levels of 250 teachers attending an online chemistry course preparing teachers for an examination to be a teacher in the public schools named as KPSS (Public Personnel Examination), toward web pedagogical content knowledge. Web Pedagogical Content Knowledge Scale developed by Lee, Tsai and Chang [1] and adapted to Turkish by Horzum [2] was used as data collection tool.