Through building the environmental monitoring curriculum system for application-oriented talents, the comprehensive design and practice were constructed from the syllabus, textbooks, web-based courses, top-quality courses, test paper bank, open laboratory and scientific research etc. The aims are to promote environmental science professional, strengthen the analysis ability, broaden the students' field of vision, optimize the environmental science major teaching, and improve multilevel integrated design and practical analysis, which ultimately cultivate the students capable of solid knowledge, theory and skills in environmental science.