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ERIC Number: EJ1117356
Record Type: Journal
Publication Date: 2016-Oct
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
A Laboratory Experiment for Rapid Determination of the Stability of Vitamin C
Adem, Seid M.; Lueng, Sam H.; Elles, Lisa M. Sharpe; Shaver, Lee Alan
Journal of Chemical Education, v93 n10 p1766-1769 Oct 2016
Experiments in laboratory manuals intended for general, organic, and biological (GOB) chemistry laboratories include few opportunities for students to engage in instrumental methods of analysis. Many of these students seek careers in modern health-related fields where experience in spectroscopic techniques would be beneficial. A simple, rapid, easily implemented experiment was developed to measure vitamin C levels in solutions using visible spectroscopy. When vitamin C is added to a mixed reagent containing Fe[superscript 3+] and 1,10-phenanthroline, the vitamin C reduces the Fe[superscript 3+] to Fe[superscript 2+] which subsequently combines with the 1,10-phenanthroline to form a reddish-orange colored complex that is measured by visible spectroscopy at 510 nm. The experiment includes measurement of the stability of aqueous vitamin C solutions when exposed to long-term storage and to heat. Students in GOB laboratory classes were able to produce results with good precision. The experiment was also extended to include the measurement of vitamin C in nutrition supplements.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Kansas
Grant or Contract Numbers: N/A