Science laboratory is a very important resource input for teaching science. Learning science is enhanced and the understanding level is improved when students are engaged in science laboratory for practical experiments. The current study aimed to assess the status of Biology laboratory and practical activities in some selected secondary and Preparatory schools of Borena Zone. A random sampling technique was employed to collect data from students, Biology teachers and technicians of the study schools. Structured and semi-structured questionnaires and observation of laboratories and other facility was used. All respondents (100%) from Kilenso School respond as there is no laboratory room while majority of respondents (80.2%) from Bule Hora School respond as they have common laboratory for each science and no separate laboratory for Secondary and Preparatory school. In all schools there is no facility, equipments and chemicals are simply stored in non-ventilated laboratory room due to absence of skilled laboratory technicians and even no cooling system. The current study is similar to report of Hunde and Tegegne (2010) in which Jimma University community school and Yebu School have laboratory which is not functional while Bilida School has no laboratory set up at all. The study is also similar to the report of Tesfamariam et al. (2014) in which most laboratory rooms available in secondary schools of Mekele town were not built for laboratory purpose and lacked even the most facility. Absence of laboratory practical activities makes students at secondary and preparatory schools of the study area lack interest to join science class.