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ERIC Number: EJ1116235
Record Type: Journal
Publication Date: 2016-Oct
Pages: 9
Abstractor: As Provided
ISSN: ISSN-1072-0502
Dopamine Receptor DOP-4 Modulates Habituation to Repetitive Photoactivation of a "C. elegans" Polymodal Nociceptor
Ardiel, Evan L.; Giles, Andrew C.; Yu, Alex J.; Lindsay, Theodore H.; Lockery, Shawn R.; Rankin, Catharine H.
Learning & Memory, v23 n10 p495-503 Oct 2016
Habituation is a highly conserved phenomenon that remains poorly understood at the molecular level. Invertebrate model systems, like "Caenorhabditis elegans," can be a powerful tool for investigating this fundamental process. Here we established a high-throughput learning assay that used real-time computer vision software for behavioral tracking and optogenetics for stimulation of the "C. elegans" polymodal nociceptor, ASH. Photoactivation of ASH with ChR2 elicited backward locomotion and repetitive stimulation altered aspects of the response in a manner consistent with habituation. Recording photocurrents in ASH, we observed no evidence for light adaptation of ChR2. Furthermore, we ruled out fatigue by demonstrating that sensory input from the touch cells could dishabituate the ASH avoidance circuit. Food and dopamine signaling slowed habituation downstream from ASH excitation via D1-like dopamine receptor, DOP-4. This assay allows for large-scale genetic and drug screens investigating mechanisms of nociception modulation.
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Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A