The purpose of the current study is to determine the pre-service science teachers' awareness levels of environmental ethics in relation to different variables. The sampling of the present study is comprised of 1,023 third and fourth year pre-service science teachers selected from 12 different universities in the spring term of 2013-2014 academic year. As a data collection instrument in the study, "Environmental Ethics Awareness Scale" developed by the researcher was employed. In the analysis of the collected data, t-test and one-way analysis of variance (ANOVA) from SPSS program package were used. The results of the t-test analysis revealed a significant difference between the male students and the female students (t[subscript (1,021)] = 4.292; p < 0.001). No significant difference was found between the third year students and the fourth year students as a result of the t-test analysis (t[subscript (1,021)] = 1.090; p > 0.05). The results of the variance analysis also did not reveal any significant difference based on the grade level (F[subscript (11-1011)] = 51.215; p < 0.001). In light of the findings of the study, it was suggested that future research should be conducted on different samplings and with different variables.