A total of 967 students (males and females) from four secondary schools in Vysocina region of Czechia were interviewed via 24-question Likert-type questionnaire to assess student's environmental awareness and perceptions. The generalized linear models were used to test if (and to what extent) student perceptions related to environment are/or not influenced by various factors including gender, age, place of residence, educational level, and specialization. The results showed that students' age, place of residence, education level and their specialization did not significantly affect (p<0.05) their environmental perceptions. However, gender appeared to be statistically significant (p<0.05) influencing student environmental perceptions and also showed linkages to basic environmental education, attitudes and engagement of students in science-related activities. Our results strongly support the need for more environmental education, awareness campaigns in the schools and engaging students in outdoor environment related activities. Future research should include detail environmental surveys targeting school students across Czechia.