The purpose of this study was to determine preservice science teachers' attitudes toward environment and to investigate whether their environmental attitudes differ in terms of gender and grade level. A total of 197 preservice science teachers participated in the study. Personal Information Form and the Environmental Attitudes Inventory (EAI) developed by Milfont and Duckitt (2006) were utilized as data collection tools in the study. The data were analyzed using the PASW Statistics 18 (SPSS Inc.). According to the results, preservice science teachers displayed moderately favorable attitudes toward environment. Furthermore, a significant gender difference favoring female preservice teachers was found in terms of total and environmental movement activism, environmental threat, and support for population growth policies dimensions of EAI. In addition, according to grade level, significant differences were found in favor of senior preservice teachers in total and human utilization of nature, and support for population growth policies dimensions of EAI. Results were discussed based on the findings obtained from the study.