Limited tools assess positive development through university sport. Such a tool was validated in this investigation using two independent samples of Canadian university athletes. In Study 1, 605 athletes completed 99 survey items drawn from the Youth Experience Scale (YES 2.0), and separate a priori measurement models were evaluated (i.e., 99 items, YES 2.0, YES for Sport [YES-S]). Confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM) results indicated issues with model fit. Post-hoc modifications improved fit, resulting in a 46-item, 9-factor model with five positive and four negative dimensions. In Study 2, 511 athletes completed the same items. The resultant model was confirmed using both CFA (comparative fit index [CFI] = 0.911, standardized root mean square residual [SRMR] = 0.056, root mean square error of approximation [RMSEA] = 0.040) and ESEM (CFI = 0.956, SRMR = 0.023, RMSEA = 0.034). The resultant University Sport Experience Survey provides a reliable and factorially valid instrument for measuring development in university sport.