The paper is devoted to monitoring the environmental coliform bacteria (CB) contamination (soil and water) in the environmental disaster areas in the Kazakhstan part of the Aral Sea Region and ranking districts by their level of contamination and the rate of gastrointestinal infections (GI). The research was done in environmental disaster areas (Aral District, Kazaly District) and environmental crisis areas (Karmakshy District, Zhalagash District, and Shieli District) in the Kyzylorda Region. The areas were ranked in terms of CB contamination level and GI rate in descending order. The bacterial composition in the gathered water samples showed that the greatest number of contaminated samples was found in the Aral District and an insignificantly smaller number of contaminated water samples were found in the Shieli District. A combination of various microorganisms (by two or three species) was found in most studied samples of soil and water, while the total microbial count ranged from 2.1 to 6.7. The obtained results show that the rankings of areas by E.coli contamination and GI rate coincided or were very close, but weakly correlated with the severity of the environmental disaster.