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ERIC Number: EJ1114217
Record Type: Journal
Publication Date: 2016
Pages: 12
Abstractor: As Provided
ISSN: ISSN-1470-8175
Regulation of Iron Acquisition Responses in Plant Roots by a Transcription Factor
Bauer, Petra
Biochemistry and Molecular Biology Education, v44 n5 p438-449 Sep-Oct 2016
The presented research hypothesis-driven laboratory exercise teaches advanced undergraduate students state of the art methods and thinking in an integrated molecular physiology context. Students understand the theoretical background of iron acquisition in the model plant "Arabidopsis thaliana." They design a flowchart summarizing the key steps of the experimental approach. Students are made familiar with current techniques such as qPCR. Following their experimental outline, students grow Arabidopsis seedlings up to the age of six days under sufficient and deficient iron supply. The Arabidopsis plants are of two different genotypes, namely wild type and "fit" loss of function mutants. "fit" mutants lack the essential transcription factor FIT, required for iron acquisition and plant growth. Students monitor growth phenotypes and root iron reductase activity in a quantitative and qualitative manner. Then, students determine gene expression regulation of "FIT," "FRO2," and a reference gene by reverse transcription-quantitative PCR (RT-qPCR). Finally, students interpet their results and build a model summarizing the connections between morphological, physiological and molecular iron deficiency responses. Learning outcomes and suggestions for integrating the course concept are explained.
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A