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ERIC Number: EJ1106051
Record Type: Journal
Publication Date: 2015
Pages: 9
Abstractor: As Provided
ISSN: ISSN-0009-2479
Ultrafiltration of Protein Solutions: A Laboratory Experiment
Pansare, Vikram J.; Tien, Daniel; Prud'homme, Robert K.
Chemical Engineering Education, v49 n1 p9-17 Win 2015
Biology is playing an increasingly important role in the chemical engineering curriculum. We describe a set of experiments we have implemented in our Undergraduate Laboratory course giving students practical insights into membrane separation processes for protein processing. The goal of the lab is to optimize the purification and concentration of a protein by hollow-fiber, tangential-flow ultrafiltration. Experiments guide the students to an appreciation for concentration polarization, i.e. the buildup of a concentrated layer of solute on the membrane surface resulting from opposing processes of convection to the membrane surface and removal from the surface by diffusion and tangential flow. The experiment draws upon concepts the students will have seen in fluid mechanics, mass transfer, and separations courses. Optimization, the process of compromising between opposing phenomena, is demonstrated by having the students design a purification and concentration process that minimizes processing time.
Chemical Engineering Education, Chemical Engineering Division of ASEE. P.O. Box 142097, Gainesville, FL 32614. Tel: 352-392-0861; Fax: 352-392-0861; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A