The aims of this study were; first to investigate the mediating effects of pre-service teachers' (PTs) attitude toward environment on the relationship between their environmental concern and environmental responsibility, and second, to explore the moderating effect of gender on the relationships between; PTs' environmental concern and responsibility; and environmental attitude and responsibility. A total of 1626 PTs completed the Environmental Literacy Survey. The findings revealed that PTs' attitude had a significant mediating effect on the relationships between environmental concern and responsibility. Furthermore, the results of the moderation analysis showed that the relationships did not differ in female and male participants.