The recent considerable developments in the field of biology have necessitated the knowledge of mathematics and its applications in biology. Therefore; most of the universities, now, believe that it is essential to include mathematics courses in the curricula of biology departments. Taking this fact into consideration, this study aims at exploring to what extent biology department students are successful in comprehending mathematical concepts and applying these in biology problems. The test administered to achieve this aim revealed that biology department students were more successful in the application of mathematics in biology.