E-content learning package will progress the learning process of students in formal or informal setting. It allows us to sort out the information to analyse and make meaning for conceptualization and applications which is suitable for individual learners. The objectives of the study was to measure the effectiveness of the E-content learning package in learning Mathematics for prospective teachers and the experimental research is essential for finding out the effective from the population of prospective teachers. The investigator selected 30 students from a college of education in Tamil Nadu. Tools used were E-content learning package for Mathematics prospective teachers developed by the investigator and pre-test in Mathematics developed by the investigator. In the experimental group, students learn the subject Mathematics by using this E-content learning package for Mathematics. The control group student teachers were taught using conventional method. Findings of the study showed that there was significant difference between pre-test and post-test scores for the experimental group in learning Mathematics. That is experimental group Prospective Teachers have achieved high in post-test than the pre-test. And also there was significant difference in the post-test scores of the experimental and control group. That is experimental group Prospective Teachers have achieved high in post-test than the control group Prospective Teachers. The investigator concludes that the experimental group is more effective than the control group. Thus E-content learning package in Mathematics Education for the prospective teachers is more effective.