Developing an attitude influential in individuals' behaviours and related with academic achievement is a concept whose development science educators consider important. This research aims to adapt the 8-item Attitude toward the Subject of Chemistry Inventory (ASCI)--which was developed by Bauer (2008) and revised by Xu and Lewis (2011)--into Turkish, and to perform the validity and reliability analyses of the Turkish form of the inventory. Confirmatory factor analysis was performed for the validity of the inventory, and Cronbach Alpha (a) coefficient as well as McDonald's Omega (?) coefficient were calculated for reliability. It was found following the confirmatory factor analysis that acceptable fit indices belonged to the model measuring two correlated different structures. It was also found in consequence of the confirmatory factor analysis that the t values were significant at the level of 0.01. Accordingly, factor loads were in the 0.31-0.84 range. Fit indices for correlated two-factor model were regarded to meet the goodness of fit criteria. Cronbach a coefficient was found to be 0.713 for the cognitive dimension where four items were included; and to be 0.731 for the affective dimension. McDonald's ? coefficient recommended for congeneric measurements was calculated as 0.74 for the cognitive dimension, and 0.75 for the affective dimension.