ERIC Number: EJ1100300
Record Type: Journal
Publication Date: 2016-Apr
Abstractor: As Provided
Peptide Mass Fingerprinting of Egg White Proteins
Alty, Lisa T.; LaRiviere, Frederick J.
Journal of Chemical Education, v93 n4 p772-777 Apr 2016
Use of advanced mass spectrometry techniques in the undergraduate setting has burgeoned in the past decade. However, relatively few undergraduate experiments examine the proteomics tools of protein digestion, peptide accurate mass determination, and database searching, also known as peptide mass fingerprinting. In this experiment, biochemistry students digest a protein mixture from egg white using the enzyme trypsin; liquid chromatography electrospray ionization time-of-flight mass spectrometry (LC--ESI-TOF-MS) separates the resulting peptides and determines their accurate masses. Instrument software is used to match these peptides to the sequences of known egg white proteins, obtained from an online source. Students then use online protein database search software to match the peptides to the protein and score the results.
Descriptors: Undergraduate Students, Biochemistry, Science Experiments, Molecular Biology, Courseware, Scientific Concepts, Scientific Principles, Web Based Instruction, Spectroscopy, Teaching Methods, Laboratory Experiments
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: firstname.lastname@example.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive; Guides - Classroom - Teacher
Education Level: Higher Education; Postsecondary Education
Sponsor: National Science Foundation (NSF)
Authoring Institution: N/A
Identifiers - Location: Virginia
Grant or Contract Numbers: CHE0922599