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ERIC Number: EJ1100277
Record Type: Journal
Publication Date: 2016-Apr
Pages: 5
Abstractor: As Provided
ISSN: ISSN-0021-9584
A Two-Week Guided Inquiry Protein Separation and Detection Experiment for Undergraduate Biochemistry
Carolan, James P.; Nolta, Kathleen V.
Journal of Chemical Education, v93 n4 p762-766 Apr 2016
A laboratory experiment for teaching protein separation and detection in an undergraduate biochemistry laboratory course is described. This experiment, performed in two, 4 h laboratory periods, incorporates guided inquiry principles to introduce students to the concepts behind and difficulties of protein purification. After using size-exclusion chromatography to separate a mixture of proteins, students utilize a colorimetric enzymatic assay and an immunoassay to determine the location of individual mixture components. Students proceed to determine the molecular weight of each protein using gel electrophoresis and generated mass spectrometric data. Completing this experiment provides students with an opportunity to expediently separate proteins while learning about protein characterization.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A