The main objective of this study is to determine the utility of simulation methods in biology teaching for nursing students and academic success. 100 students (50 control, 50 experimental) who studied at Srinivasa Teacher Training School, Kalikiri, Recognised by Sri Venkateswara University, Faculty of Education, Tirupati, AP, India, 2014-215 academic year participated in this research. In the present study mixed methods approaches were used namely qualitative and quantitative methods. Instructional transaction included the subject of "Biology" in the "daily life and importance" unit. Statistical results (p = 0.01, p<0.001, and p<0.001) of the present study data after obtained the application revealed that the experimental students group were more successful who were taught by simulation method than the students of the control group taught by traditional approaches. Once again the results of the present study evidenced that experimental students who taught by using simulation method were satisfied with simulation-based biology education.