The main purpose of the paper was to study the students' perception towards teachers' depth of content knowledge, teachers' skill of using instructional strategies, diagnosis of learning difficulties and teachers' knowledge of students' understanding in relation to their professional health. The students' of different Indian Universities were the population of the study. Among them 2,000 students and 500 University teachers from the population were the selected sample of the study. All the samples were collected by following random sampling techniques. The present study was a survey by means of enquiring the present status of learners' perception of teachers' depth of content knowledge, teachers' skill of using instructional objective, diagnosis of learning difficulties and knowledge of students' understanding as the instruments for professional health check up at University level. It indicated that there was a positive significant relationship between all the factors above. It was resulted that students' perception on these factors were the predictors of teachers' professional health check-up. Therefore, teachers should be aware and they should increase their overall depth of knowledge for the flexible teaching-learning process. It is necessary to conduct investigation regarding all teachers' depth of knowledge, academic career and the relation with age and gender.