The associations between parenting practices and adolescent anxiety symptoms were examined in both individual and monozygotic (MZ) twin differences levels. Participants were 804 pairs of Chinese MZ adolescent twins aged 10-18 years (M = 13.57, SD = 2.67, 52% females). Twins' anxiety symptoms were assessed by self- and parent-reports. Twins also reported their perceived parenting practices. On the individual level, parental warmth-reasoning was negatively, whereas harshness-hostility was positively, associated with both self- and parent-reported youth anxiety. On the MZ-twin differences level, the magnitudes of the associations between parenting practices and youth anxiety were decreased. MZ-twin difference in parental warmth-reasoning remained significantly associated with self- and parent-reported youth anxiety; MZ-twin difference in parental harsh-hostility was only significantly associated with self-reported youth anxiety. This study indicated that parental warmth-reasoning and harshness-hostility may be unique environmental experiences that influence youth anxiety, and illustrated the necessity of controlling for gene-environment correlations when examining the true environmental effects of parenting on child behavior.