The purpose of the present research is to define global citizenship levels of pre-service physical education teachers and investigate whether their global citizenship levels vary by various variables. A total of 485 pre-service teachers, studying at 3rd and 4th grades of undergraduate programs of physical education teaching at thirteen different universities participated in the present research. The research is a descriptive study in survey model. In order to collect data, Global Citizenship Attitude Scale, developed by Sahin and Çermik was utilized. Obtained data were analysed through descriptive statistics (Mean, standard deviation), t-test, ANOVA test, and finally Tukey test for multiple comparisons. According to the findings obtained in the present research, global citizenship of preservice physical education teachers is medium level and can be developed; additionally global citizenship level does not vary by gender and YGS (The Transition to Higher Education Examination) scores; while increase in total family income, daily Internet use and foreign language proficiency increased global citizenship level, but not at a statistically significant level. An interesting finding of the present research is that, the university, the number of foreign friends, and having athlete licence variables are effective on global citizenship level.