Fitness centers require professional instructors to ensure members obtain the safest and most effective exercise classes. Certification and licensure enable fitness instructors to demonstrate a strong desire to be knowledgeable and committed to expand their capabilities and offer more diverse services to their clientele. This study therefore sought to determine whether fitness instructors in the Greater Accra Region of Ghana had the needed professional competence required to be effective as instructors. A descriptive correlational research design was used for the study. A total of 18 instructors were purposively selected from an accessible population of 45. A researcher designed appraisal questionnaire was used to gather data for the study. Three hypotheses were tested using Pearson Product Moment Correlation Coefficient at 0.05 level of significance. The findings showed no significant relationship existed between highest academic qualification (HAQ) ("r" = 0.427, p = 0.077), area of study (AoS) ("r" = -0.385, p = 0.115, as well as years of experience (YoE) and level of knowledge ("r" = 0.192, p = 0.444) all 2-tailed. It was concluded that academic qualification, area of study and years of experience did significantly relate with the level of knowledge suggesting that the instructors' lack of training, certification and licensure culminated into their low levels of current knowledge in fitness instruction. It was recommended that the National Association for all Sports, Ghana and other stakeholders should develop a national plan for the training, certification and licensure of Fitness Instructors in Ghana.