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ERIC Number: EJ1090635
Record Type: Journal
Publication Date: 2016-Feb
Pages: 3
Abstractor: As Provided
ISSN: ISSN-0021-9584
Measuring Norfloxacin Binding to Trypsin Using a Fluorescence Quenching Assay in an Upper-Division, Integrated Laboratory Course
Hicks, Katherine A.
Journal of Chemical Education, v93 n2 p380-382 Feb 2016
Fluorescence quenching assays are often used to measure dissociation constants that quantify the binding affinity between small molecules and proteins. In an upper-division undergraduate laboratory course, where students work on projects using a guided inquiry-based approach, a binding titration experiment at physiological pH is performed to analyze the interaction between the drug norfloxacin and the protein trypsin. The resulting nonlinear binding data are fitted using a scientific data analysis program to determine the dissociation constant (K[subscript d] value) describing this interaction. The experiment is especially of interest to students who plan to pursue careers in health-related aspects of biochemistry.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A