ERIC Number: EJ1090635
Record Type: Journal
Publication Date: 2016-Feb
Pages: 3
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Measuring Norfloxacin Binding to Trypsin Using a Fluorescence Quenching Assay in an Upper-Division, Integrated Laboratory Course
Hicks, Katherine A.
Journal of Chemical Education, v93 n2 p380-382 Feb 2016
Fluorescence quenching assays are often used to measure dissociation constants that quantify the binding affinity between small molecules and proteins. In an upper-division undergraduate laboratory course, where students work on projects using a guided inquiry-based approach, a binding titration experiment at physiological pH is performed to analyze the interaction between the drug norfloxacin and the protein trypsin. The resulting nonlinear binding data are fitted using a scientific data analysis program to determine the dissociation constant (K[subscript d] value) describing this interaction. The experiment is especially of interest to students who plan to pursue careers in health-related aspects of biochemistry.
Descriptors: Science Instruction, Science Laboratories, Undergraduate Study, College Science, Chemistry, Spectroscopy, Inquiry, Biochemistry, Science Experiments, Laboratory Experiments, Scientific Concepts
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A