This paper aims at investigating the negative particles and morphemes in three main Jordanian dialects (Urban, Rural and Bedouin). This quantitative and qualitative study includes 30 teachers from different disciplines who use these dialects. The sample of the study was selected randomly. The research used two research instruments, a checklist and an interview. The findings of this research indicate that there are six particles for negation in the three Jordanian dialects and one negative morpheme. This study recommends that other research be conducted to further examine relevant expressions used for negation in these dialects and other negative particles and morphemes among other Arabic language dialects such as Syrian, Egyptian, Saudi and Moroccan.