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ERIC Number: EJ1084333
Record Type: Journal
Publication Date: 2015-Dec
Pages: 6
Abstractor: As Provided
ISSN: ISSN-0021-9584
On-Line Synthesis and Analysis by Mass Spectrometry
Bain, Ryan M.; Pulliam, Christopher J.; Raab, Shannon A.; Cooks, R. Graham
Journal of Chemical Education, v92 n12 p2146-2151 Dec 2015
In this laboratory experiment, students learn how to use ESI to accelerate chemical synthesis and to couple it with on-line mass spectrometry for structural analysis. The Hantzsch synthesis of symmetric 1,4-dihydropyridines is a classic example of a one-pot reaction in which multiple intermediates can serve to indicate the progress of the reaction as a function of the electrospray variable parameters. This reaction allows students to gauge the effects of the spray variables while observing the enhanced reaction rate through product analysis in the charged microdroplets. Soft ionization techniques used in conjunction with collision-induced dissociation provide students experience with full scan MS to infer reaction progress and collision-induced fragmentation patterns for structural analysis. The experiment as a whole combines the physical phenomena of electrospray, Hantzsch chemical reactivity, and analytical measurements involving use of an ion trap mass spectrometer.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Science Foundation (NSF)
Authoring Institution: N/A
Identifiers - Location: Indiana
Grant or Contract Numbers: CHE1307264