The construct of anxiety plays a major role in one's life. One of these anxieties is test anxiety or apprehension over academic evaluation. The present study was designed to investigate the relationship between gender, academic achievement, years of study and levels of test anxiety. This investigation is a descriptive analytic study and was done on 110 undergraduate students from University of Isfahan. The Suinn's Test Anxiety Questionnaire with 48 questions was used to gather the data. For analysis of data correlation coefficient and chi square test were used. The findings revealed that female students have a higher level of test anxiety in contrast to male students. The average of test anxiety scores among female students was higher. Also a statistically significant negative correlation was observed between test anxiety and academic achievement. There was no meaningful relationship between test anxiety and years of study.