Objectives: The present study examined dietary intake of fruit and vegetables and dietary patterns of preadolescents attending schools in the Midwest. Methods: A total of 506 students (11.2 ± 1.3 years) from four public and private schools in Nebraska completed a validated 41-item Food Frequency Questionnaire to assess their dietary intake. Cluster analysis was used to cluster participants into low and high fruit and vegetable intake groups based on their responses to survey items associated with fruit and vegetable intake. Results: The majority of the students (>50%) reported that their intake of each of the fruit and vegetable categories was less than three days a week. Males had significantly lower intake of most of the fruit and vegetable categories and whole grains compared to females (p<0.05). Students with higher fruit and vegetable intake also had higher intake of dairy, whole grains, and protein foods as well as higher intake of low nutritional quality foods compared to those with low fruit and vegetable intake (p<0.05). No significant difference was found in dietary intake of sugar sweetened foods between high and low fruit and vegetable intake groups (p = 0.50). Applications to Child Nutrition Professionals: Continued nutrition education and implementation of school nutrition policies to create a healthy food and nutrition environment in the school and at home are important for healthy eating among Midwestern children.