In the light of the problems existing in assessment of the course of "athletic physiology" in universities at present, this article makes reflections upon the assessment mode of students for this course and takes a general survey on development of the evaluation system by comprehensively employing both the quantitative method and qualitative method. By taking into consideration of educational evaluation, it focuses on diversification, process, development and practice and sets up a set of new system of assessment and evaluation with combination of "theory, design and application", which is of great significance to promote curriculum reform of "athletic physiology", improve teaching quality and exam style and learning style as well as fulfill the teaching goal of the course.