The current study aims to examine the effect of interactive e-books on students' achievement at Najran University in computer in education course. Quasi-experimental study design is used in the study and to collect data the researchers built achievement test to measure the dependent variable represented in the achievement affected by experimental treatment. The study sample is (60) students from the fifth level at Najran University in Saudi Arabia who registered in computers in education course, which has been divided into two groups, each with a strength (30) student, experimental group students study the computer in education course via e-book, and the control group students study the computer in education course via printed-book. The findings of the study illustrate there is a significant difference between the study groups in academic achievement favor to experimental group students who study with e-book.