This study aims to examine the enhancement of mathematical reasoning ability through reflective learning. This study used quasi-experimental method with nonequivalent pretest and posttest control group design. The subject of this study were students of Mathematics Education Program in one of private universities in Palembang, South Sumatera, Indonesia, consisting of 155 students. Subject of study are differentiated into two groups namely experimental group who were taught by reflective learning (RL) and control group who were taught by conventional learning (CL). This study was conducted in odd semester academic year of 2013/2014. Instrument in this study are mathematical reasoning ability test, observation sheet, and interview guide. By using Mann-Whitney test, it is concluded that achievement and enhancement of mathematical reasoning ability of student teachers who received RL is better than student teachers who received CL.