Background: Organizational factors are considered to be the cornerstone in achieving psychological and professional security at work, which in turn are positively reflected in job performance both quantitatively and qualitatively. Aim of the Study: The study aimed to assess night shift and education/training developmental factors on performance of professional nurses in north West Bank governmental hospitals. Subjects and methods: A quantitative descriptive study utilized stratified random sampling of 185 nurses. A self administered questionnaire was developed with a response rate of 97%. The questionnaire was validated by experts, and reliability was obtained by Cronbach's alpha coefficient was (0.86) Data were analyzed using SPSS. Results: The overall level of night shift factor affecting performance of professional nurses was high (79.0%) and ; the education/training developmental factors moderate (68.8%) as perceived by nurses. It was found that there are significant differences at a = 0.05 between the total score of night shift and education/training developmental factors towards workplace in favor to Dr. Nazal Hospital. Conclusion and recommendation: The night shift and education/ training developmental factors affected the performance of the professional nurses. The findings of this study provide recommendations and suggestions to improve the performance of professional nurses in governmental hospitals such as: reduce the strain of long work hours on night shift and motivation. Providing continuous professional development of nurses in service training program.