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ERIC Number: EJ1074537
Record Type: Journal
Publication Date: 2015
Pages: 8
Abstractor: As Provided
ISSN: ISSN-1470-8175
A Semester-Long Project-Oriented Biochemistry Laboratory Based on "Helicobacter pylori" Urease
Farnham, Kate R.; Dube, Danielle H.
Biochemistry and Molecular Biology Education, v43 n5 p333-340 Sep-Oct 2015
Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically relevant enzyme--"Helicobacter pylori" ("Hp") urease--the activity of which is absolutely required for the gastric pathogen "Hp" to colonize the human stomach. Over the course of the semester, students undertake a biochemical purification of "Hp" urease, assess the success of their purification, and investigate the activity of their purified enzyme. In the final weeks of the semester, students design and implement their own experiments to study "Hp" urease. This laboratory provides students with an understanding of the importance of biochemistry in human health while empowering them to engage in an active area of research.
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Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: National Center for Research Resources (NIH/DHHS); National Institute of General Medical Sciences (NIH)
Authoring Institution: N/A
Grant or Contract Numbers: 5P20RR016463-12; 8 P20 GM103423-12