ERIC Number: EJ1074514
Record Type: Journal
Publication Date: 2015-Sep
Abstractor: As Provided
Reference Count: 26
Isothermal Titration Calorimetry and Macromolecular Visualization for the Interaction of Lysozyme and Its Inhibitors
Wei, Chin-Chuan; Jensen, Drake; Boyle, Tiffany; O'Brien, Leah C.; De Meo, Cristina; Shabestary, Nahid; Eder, Douglas J.
Journal of Chemical Education, v92 n9 p1552-1556 Sep 2015
To provide a research-like experience to upper-division undergraduate students in a biochemistry teaching laboratory, isothermal titration calorimetry (ITC) is employed to determine the binding constants of lysozyme and its inhibitors, N-acetyl glucosamine trimer (NAG[subscript 3]) and monomer (NAG). The extremely weak binding of lysozyme/NAG is determined using a competitive binding assay. Such interactions between lysozyme and its inhibitors are visualized with PyMol software, by which the hydrogen bond formation in the complexes is used to explain the binding specificity. The hydrogen bond inventory in the binding interface correlates with the heat enthalpy determined or derived from ITC measurements. A possible explanation for such a correlation is presented and used for an extensive discussion in thermodynamics and ligand-receptor interactions. This laboratory exercise stimulates students' critical thinking about weak/strong binding interactions and the relationship between thermodynamics and structural changes.
Descriptors: Undergraduate Students, Thermodynamics, Biochemistry, Science Laboratories, Laboratory Experiments, Science Experiments, Hands on Science, Science Instruction, Visualization, Computer Software, Correlation, Laboratory Equipment, Measurement Equipment
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: email@example.com; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Sponsor: National Science Foundation (NSF)
Authoring Institution: N/A
IES Grant or Contract Numbers: DUE-0941517