This article builds upon the experiences and results of the application of sustainability assessment instruments in higher education. First, a short overview of existing sustainability assessment tools will be provided, as well as their contribution towards the integration of sustainability in higher education. Second, a reflective case focuses on the use of sustainability assessment for policy development in higher education. This case is based upon the application of the Auditing Instrument for Sustainability in Higher Education in a Belgian higher education institution, focusing on four consecutive assessments in the department of Business Studies (2003, 2004, 2009, 2010). Based on the assessment reports and an evaluation of the assessments, the contribution of sustainability assessment to policy development is reflected upon. The article provides a research-based, reflective study, and focuses on the consecutive application of sustainability assessment tools, the contribution of sustainability assessment to policy development and the contribution of sustainability assessment to connecting a bottom-up approach to a top-down approach. The article results in specific recommendations on the use of sustainability assessment for policy development in higher education.